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In my case I am very lucky as my wife does all the breeding of rats & mice etc for my collection. We have found CO2 very effective & humaine, with mice & rats they normally pass out within 30sec to 1 min, we have had many problems though with pinky mice as you can go 20 mins & they are still kicking & we also found that if we wacked them (I stress the least preffered method) that still does not work as it can take many attempts as it is very hard to get a good hold of them. Now with big rats we have found that wacking them against something to be the worst method as the skin around the tail tends to come off & leave the rodent in pain so I agree with a previous post that holding the rear legs may be the way to go.
And to the people who think the freezer is the way to go - what a cruel thing to do, even though my wife breeds the rodents for feeding for my collection she still treats them like pets to her & with that extra care & food etc we do end up at the end of the day with higher quality food for my snakes & no pain to the rodent.
Thanks
Jason
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